Expresión de una proteína relacionada al receptor de estrógeno (p29), en carcinoma primario de la mama: análisis metodológico, anatomoclínico y del contenido de ADN / Expression of a estrogen related protein (p29) in primary breast carcinoma: methodological, anatomoclinical and DNA contents analysis

The aim of this work was to assess the immunohistochemical detector of a estrogen receptor related protein (p29) in 48 histological samples of primary mammary carcinoma and its relationship to clinical, morphological and ADN content parameters. p29 protein was positive in 62.5 per cent of samples. Over 50 per cent of samples had moderate or intense immunohistochemical staining (staining index over 16) and 77 per cent has a heterogenous expression of p29 protein. Sevently six per cent of p29 positive and 53 per cent of p29 negative tumors had a proliferation fraction over 10 per cent (determined by the fraction with flux cytometry). No relationship between p29 expression and the analyzed anatomoclinical variables was found. These results highlight this immunohistochemical method as an alternative to more complex and difficult biochemical thechniques. On the other hand the good results obtained in formalin fixed tissues allow retrospective studies in mammary carcinoma samples

Cancer de mama y citometría de flujo: estudio comparativo del patrón de ploidia de ADN con parámetros anatomoclínicos / Breast cancer and flow cytometry: comparative study of DNA ploidy pattern with anatomoclinical parameters

There is evidence that DNA quantization and histopathological classification of breast cancer may be useful for its therapeutic management. DNA flow cytometry clinical and anatomopathological features of 60 paraffin embedded primary breast cancer tissue samples were studied. The aneuploidy percentage was 67 per cent . There was a correlation between DNA index and degree of cellular pleomorphism, degree of diferentiation and the fraction of cells in S phases. Likewisw a correlation was found between the degree of cellular pleomorphism and the mitotic index. DNA cytophotometry was useful to solve cases of difficult diagnosis with flow between aneuploidy, percentage of cells in phase S, degree of cellular atypia and mitotic degree with clinical stage, degree of lymph node involvement, tumoral size or age. It is suggested that these variables may have an independent behavior